Customization: | Available |
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CAS No.: | 367-93-1 |
Formula: | C9h18o5s |
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Description |
Isopropyl-β-D-thiogalactoside, often used in combination with X-Gal or Bluo-Gal, is used for blue and white screening of recombinant l colonies. IPTG is not metabolized by and is a lactose analogue. Once it enters the cell, it will produce a long-lasting induction effect. Therefore, the induction efficiency of IPTG is high, and often only a small amount (1 mmol/L) is needed to achieve the ideal induction effect. Since IPTG is not metabolized, it specifically induces the expression of foreign proteins in the cell. Unaffected by cell metabolism, the induction effect remains stable. |
Function |
Commonly used molecular biological reagents, such as blue and white spot screening and IPTG induced protein expression in bacteria, etc IPTG is an effective inducer of protein expression in the concentration range of 100 μM to 1.0 mM. Concentration used depends on the strength of induction required, as well as the genotype of cells or plasmid used. If lacIq, a mutant that over-produces the lac repressor, is present, then a higher concentration of IPTG may be necessary. In blue-white screen, IPTG is used together with X-gal. Blue-white screen allows colonies that have been transformed with the recombinant plasmid rather than a non-recombinant one to be identified in cloning experiments. |
Application |
1.Beta-galactosidase inducer.Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology. 2.Isopropyl-beta-D-thiogalactopyranoside is used as an inducer for the activity of E. Coli lac operon. |
VC-IP/Ascorbyl Tetraisopalmitate | Tranexamic Acid | 1,4-Dipropionyloxybenzene |
Alpha Arbutin | Ectoin | Glycyrrhetinic Acid (18-Beta Glycyrrhetinic Acid) |
Beta Arbutin | Dimethylmethoxy Chromanol | Dimethymethoxyl Chromanyl Palmitate |
Deoxyarbutin | Alpha Lipoic Acid | Magnesium Ascorbyl Phosphate |
Kojic Acid | Charcoal Powder(Bamboo) | Sodium Ascorbyl Phosphate |
Kojic Acid Dipalmitate | Stearyl Glycyrrhetinate | Hydrocotyle Asiatica Extract |
Ergothioneine | Tetrahydrocurcumin | Potassium 4-Methoxysalicylate |
Carnosine | Glycolic Acid | Salicylic Acid |
Undecylenoyl Phenylalanine | Bromelain | Alpha Hydroxy Acid/AHA |
Caffeic acid | Mequinol | Phytic acid |
Niacinamide | SymWhite Powder | Snow White |
Papain | 3-o-ethyl ascorbic acid/VCE | Giga White |
Monobenzone | Mandelic acid | Glabridin |
Azelaic Acid | Melatonin | Dipotassium Glycyrrhizinate |
Ascorbyl Glucoside/AA2G | L-Glutathione Reduced | Procysteine |
Fullerence C60 | Cetyl Tranexamate HCl/TXC | Hydroxypinacolone reinoate(HPR) |
Sodium Hyaluronate/Hyaluronic Acid | Beta-Glucan | Sodium Polyglutamate | Inositol |
Pro-xylane/Hydroxypropyl tetrahydropyrantriol | Collagen | 2-O-β-D-glucosyl-glycerol | Glucosylglycerol |
Biosaccharide Gum-1 | Ceramide | Orotic Acid Monohydrate | 1,2-Hexanediol |
Sodium L-pyroglutamate;PCA-Na | D-panthenol | Maltobionic Acid | Lactobionic acid |
Caprylyl Glycol/1,2-Dihydroxyoctane | DL-panthenol | Allantoin | Polyglutamic Acid |
Ascorbic Acid (Vitamin C) | Magnesium Ascorbyl Phosphate | Ascorbyl Isostearate | Ascorbyl Glucoside |
3-o-Ethyl Ascorbic Acid | Sodium Ascorbyl Phosphate | Ascorbyl Tetraisopalmitate | Ascorbyl Palmitate |
Apple Polyphenols | Sodium Copper Chlorophyllin | D-Isoascorbic Acid | Ferulic Acid | EGF/Oligopeptide-1 |
Ascorbyl Palmitate | Resveratrol | Vitamin E | Copper PCA | Vitamin E |
EUK 134 | Glucosylrutin | Pterostilbene | ZINC PCA | Coenzyme Q10 |
Vanillyl butyl ether | Menthol Crystal | Menthyl lactate | Menthone 1,2-glycerol ketal |
Capsaicin | WS-23 | Camphor | WS-3 |
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